Kenneth C. Ingham, PhD
B.S. in Chemistry & Mathematics, Eastern Michigan University, 1965
Ph.D. in Physical Chemistry, University of Colorado, 1970
1970-1972 Fellow, Depts of Biophys & Biochem, Michigan State U
1972-1975 Sr. Fellow, National Institutes Health (NIAMD)
1975-2004 Scientist, Am Red Cross Blood Services, Bethesda, MD
1983-1984 Visiting Scientist, Biozentrum, U of Basel, Switzerland
1985-2004 Head, Biochem Dept, Am Red Cross, Rockville, MD
1988-2004 Prof of Biochem and Molec Biol, George Washington U
Fogarty International Fellowship, 1983-84,
Research Career Development Award, NHLBI, 1977-82
American Society of Biological Chemists
International Society of Thrombosis and Hemostasis
The Protein Society
International Society of Biorecognition Technology
External Appointments and Committees:
Molecular and Cellular Biophysics Study Section, NIH (l981-85)
Ad Hoc, Biomed Science Fellowship Review, NIH (1980;1983)
Site-Visitor, NHLBI Prog Proj Review, Temple University, Thrombosis & Hemostasis,
Site-Visitor, NIGMS Prog Proj Review, Albany Medical Center, Tissue Injury and Repair,
Faculty Member, Fdn. Adv. Educ. Sciences, NIH (1978-83)
Member, Molecular Sciences Review Panel, NSF, 1989
Exchange Scientist, Inst. of Biochemistry, Ukranian Academy of Science, Kiev (1989;1991)
Member, Biochemistry of Fibrinolysis Study Section, NIH, 1990
Site-Visitor, NHLBI Prog Proj Review, U of Wisc Milwaukee, Structure & Function of
Clotting Proteins, 1991
Site-Visitor, NHLBI Prog Proj Review, U of Rochester, Basic and Clinical Studies of
Coagulation Proteins, 1993
Ad Hoc, Molecular and Cellular Biophysics Study Section, NIH, 1994
Ad Hoc, Heme-1 Study Section, NIH (1995)
Site-Visitor, NIGMS Prog Proj Review, Albany Medical Center, Wound Healing, 1996
Member Editorial Board, Archives of Biochemistry Biophysics, 1998-2004
Chair, Seminar Committee, ARC Blood Services (1976-1981; 92-93; 97-98)
Chair, Plasma Fractionation Task Force, ARC Blood Services (1977-82)
Chair, Viral Inactivation Task Force, ARC Blood Services (1981-84)
Chair, Radiation Safety Committee, ARC Holland Labs (1988-91)
Member, Vivarium Advisory Committee, ARC Holland Labs (1998-00)
Subunit Interactions in Glycoprotein Hormones, NIAMDD (1977-80)
Precipitation of Proteins with Poly(ethylene glycol),NHLBI (1977-80)
Physical Chemistry of Therapeutic Plasma Proteins, NHLBI (1981-91)
Shared Instrumentation Grant, NHLBI (1988)
Domain Structure of Plasma Proteins, Mathers Fndn (1990-95)
Molecular Interactions of Fibronectin, NHLBI (1990-95)
Protein and Nucleic Acid Core Facility, NHLBI (1990-95)
Domain Structure and Interactions of Fibronectin, NHLBI (1992-97)
Domain Structure of Integrin IIbIIIa, Fogarty Internat Cntr (1993-96)
Interactions of Fibronectin Modules, NHLBI (1997-2002)
1. Ingham, K.C. and Strickler, S.J. (1970) Hindered Internal Rotation in the Ground and
Excited Electronic States of Ortho-Xylene, J. Chemical Physics 53,4313- 4318.
3. Ingham, K.C. (1971) The Role of Non-Bonded Forces in Barriers to Internal Rotation in
Molecules with Two Equivalent Methyl Groups, J. Phys. Chem. 76,551-553.
6. Ingham, K.C. and El-Bayoumi, M.A. (1974) Photo-induced Double Proton Transfer in a
Model DNA Base Pair; J. Am. Chem.
11. Ingham, K.C., Saroff, H. and Edelhoch, H. (1975) Ligand Induced Self-Association of
Human Chorionic Gonadotropin: Positive Cooperativity in the Binding of 1,8-
Anilinonaphthalene Sulfonate, Biochemistry 14, 4751-4758.
13. Ingham, K.C., Weintraub, B.D. and Edelhoch, H. (1976) Kinetics of Recombination of
the Subunits of Human Gonadotropin. Effect of Subunit Concentration, Biochemistry
19. Miekka, S.I. and Ingham, K.C. (1978) Influence of Self-Association of Proteins on Their
Precipitation by Poly(ethylene glycol), Arch. Biochem. Biophys. 191, 525-536.
20. Busby, T.F. and Ingham, K.C. (1980) Separation of Macromolecules by Ultrafiltration: J. Biochem. Biophys. Methods 2, 191-206.
29. Atha, D. and Ingham, K.C. (1981) Mechanism of Protein Precipitation by Polyethylene
Glycol: Analysis in Terms of Excluded Volume, J. Biol. Chem. 256, 12108-12117.
32. Forastieri, H. and Ingham, K.C. (1982). Thermal Stability of Human Gonadotropins:
Reversible Dissociation of Subunits at Neutral pH, J. Biol. Chem. 257, 7976-7981.
36. Forastieri, H. and Ingham, K.C. (1983) Fluid-Phase Interaction between Human Plasma
Fibronectin and Gelatin Determined by Fluorescence Polarization, Arch. Biochem. Biophys.
41. Ingham, K.C., Brew, S.A. Broekelmann, T.J. and McDonald, J.A. (1984) Thermal
Stability of Human Plasma Fibronectin and Its Constituent Domains, J. Bio. Chem. 259,
44. Ingham, K.C., Landwehr, R. and Engel, J. (1985) Interaction of Fibronectin with C1q and
Collagen: Effects of Ionic Strength and Denaturation of the Collagenous Component. Eur.
J. Biochem. 148, 219-224.
47. Lennick, M., Brew, S.A. and Ingham, K.C. (1986) "Kinetics of Interaction of C1-Inhibitor
with Complement C1, Biochemistry 25, 3890-3897.
48. Busby, T.F. and Ingham, K.C. (1987) Calcium Sensitive Thermal Transitions and
Domain Structure of Human Complement Subcomponent C1, Biochemistry 26, 5564-5571.
54. Busby, T.F. and Ingham, K.C. (1990) The amino terminal calcium-binding domain of
human complement C1s mediates the interaction of C1r with C1q, Biochemistry 29, 4613-
57. Litvinovich, S., Strickland, D., Medved, L. and Ingham, K. (1991) Domain Structure and
interactions of the type I and type II modules in the gelatin-binding region of fibronectin:
All six modules are independently folded, J. Mol. Biol. 217, 563-575.
58. Novokhatny, V.V., Ingham, K.C. and Medved, L.V. (1991) Domain structure and
domain-domain interactions of recombinant tissue plasminogen activator, J. Biol. Chem.
63. Ingham, K.C., Milasincic, D.J., Busby, T.F. and Strickland, D.K. (1992) Dynamic
Equilibria between subcomponents of C1, the first component of human complement,
Molecular Immunology 29, 45-51.
69. Ward, S.L. and Ingham, K.C. (1992) A calcium-binding monoclonal antibody that
recognizes a non-calcium-binding epitope in the short consensus repeat units (SCRs) of
complement C1r, Mol. Immunol. 29, 83-93.
73. Radek, J.T., Jeong, J.-M., Murthy, S.N.P., Ingham, K.C. and Lorand, L. (1993) Affinity of
human erythrocyte transglutaminase for a 42-kDa gelatin-binding fragment of human
plasma fibronectin, Proc. Natl. Acad. Sci. USA 90, 3152-3156.
76. Ingham, K.C., Brew, S.A. and Migliorini, M. (1994) An unusual heparin-binding
peptide from the carboxy-terminal hep-2 region of fibronectin, Arch. Biochem. Biophys.
80. Rivas, G., Ingham, K.C. and Minton, A.P. (1994) Ca2+-linked association of human
complement Cls and Clr, Biochemistry 33, 2341-2348.
85. Litvinovich, S.V. and Ingham, K.C. (1995) Interactions between type III domains in the
110 kDa cell-binding region of fibronectin, J. Mol. Biol. 248, 611-626.
88. Busby, T.F., Argraves, W.S., Brew, S.A., Pechik, I. Gilliland, G.L. & Ingham, K.C. (1995)
Heparin binding by fibronectin module III-13 involves six discontinuous basic residues
brought together to form a cationic cradle, J.Biol.Chem. 270,18558-18562.
93. Ingham, K.C., Brew, S.A., Huff,S., & Litvinovich, S.V. (1997) Cryptic self-association
sites in type III modules of fibronectin, J.Biol.Chem. 272, 1718-1724.
97. Litvinovich,S.V., Brew,S.A., Aota,S., Akiyama, S., Haudenschild,C., and Ingham, K.C.
(1998) Formation of amyloid-like fibrils by self-association of a partially unfolded
fibronectin type III module, J. Mol. Biol. 280,245-258.
99: Bloom, L., Ingham, K.C. and Hynes, R.O. (1999) Fibronectin regulates assembly of actin
filaments and focal contacts in cultured cells via the heparin-binding site in repeat III13,
Mol. Biol. Cell 10, 1521-1536.
100. Yakovlev, S., Makogonenko, E., Kurochkina, N., Nieuwenhuizen, W., Ingham, K. and
Medved, L. (2000) Conversion of fibrinogen to fibrin: mechanism of exposure of tPA- and plasminogen-binding sites, Biochemistry 26, 15730-15741.
102. Ingham, K.C., Brew, S.A. and Migliorini, M (2002) Type I collagen contains at least 14
cryptic fibronectin binding sites of similar affinity, Arch. Biochem. Biophys. 407, 217-223.
103. Katagiri, Y., Brew, S.A. and Ingham, K.C. (2003) All six modules of the gelatinbinding
domain of fibronectin are required for full affinity, J. Biol. Chem. 278.11897-11902.
105. Ingham, K.C., Brew, S., Vaz, D., Sauder, D.N., McGavin, M.J. (2004) Interaction of
Staphylococcus aureus fibronectin-binding protein with fibronectin: affinity,
stoichiometry, and modular requirements, J. Biol. Chem. 279,42945-42953.
106. Makogonenko, E., Ingham, K.C., and Medved, L. (2007) Interaction of the fibronectin
COOH-terminal Fib-2 regions with fibrin: further characterization and localization of the
Fib-2-binding sites, Biochemistry 46, 5418-5426.